Biologically Relevant Murine Models of Chronic Pseudomonas aeruginosa Respiratory Infection

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen and the leading cause of infection in patients with cystic fibrosis (CF). The ability P. to evade host responses develop into chronic causes significant morbidity mortality. Several mouse models have been developed study respiratory infections induced by aeruginosa, bead agar model being most widely used. However, this has several limitations, including requirement for surgical procedures high mortality rates. Herein, we describe novel adapted biologically relevant lung caused aeruginosa. Three methods are described: a clinical isolate model, utilising isolates obtained from CF; incomplete antibiotic clearance bacterial bounce-back; establishment infection; water bottle model. These circumvent procedure and, importantly, can be wild-type mice. We also demonstrate successful induction transgenic βENaC murine CF. envisage that described will facilitate investigations microbial factors, efficacy antimicrobials, during infections.